| Pierce SILAC Protein Quantitation Kits for identification, characterization and quantification of the proteome launched by Thermo Fisher Scientific |
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November 28 2007 |
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Chromatography and Mass Spectrometry |
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Thermo Fisher Scientific Inc., the world leader in serving science introduces Thermo Scientific Pierce SILAC Protein Quantitation Kits for comprehensive identification, characterization and quantitation of proteins. The new kits allow for isotopic labeling of the proteome for the analysis of relative protein abundance by mass spectrometry (MS), providing an all-in-one solution for the convenience of researchers in academia, pharmaceutical and biotechnology industries. The Pierce SILAC (Stable Isotope Labeling with Amino Acids in Cell Culture) Protein Quantitation Kits provide significant advantages to proteomics researchers, including in vivo labeling that incorporates 13C- or 15N-labeled amino acids directly into proteins using normal cellular metabolism of living cells. This strategy provides 100% label incorporation into proteins prior to digestion. These kits include Thermo Scientific HyClone® Media, heavy and light amino acids, and HyClone Dialyzed Serum in a single convenient package.
The Pierce SILAC Kits enable quantitation of protein expression levels between differentially treated cell populations. This is made possible because the heavy and light amino acids are chemically identical to each other, yet isotopically distinct. By employing high resolution analysis with the Thermo Scientific LTQ-FT Ultra and LTQ Orbitrap, the differentially expressed proteins are detected by high resolution MS analysis based on relative peak intensity.
The Pierce SILAC Kits are compatible for use with mammalian cell lines adapted to grow in either HyQ® DMEM or RPMI-1640 media, including HeLa, 293T, COS7, U2OS, A549, A431, Hep-G2, NIH 3T3, Jurkats and others. When combined with Thermo Scientific Protein/Peptide Sample Enrichment Products, the Pierce SILAC Protein Quantitation Kits also enable MS analysis of low-abundance, cell-surface and organelle-specific proteins and post-translational modifications such as phosphorylation or glycosylation.
For more information on the Thermo Scientific Pierce SILAC Protein Quantitation Kits, please call +1 800-874-3723, e-mail Pierce.TS@thermofisher.comor visit www.thermo.com/pierce. Thermo Fisher delivers MS innovation to every laboratory.
Thermo Scientific is part of Thermo Fisher Scientific, the world leader in serving science.
About Thermo Fisher Scientific
Thermo Fisher Scientific Inc. (NYSE: TMO) is the world leader in serving science, enabling our customers to make the world healthier, cleaner and safer. With an annual revenue rate of more than $9 billion, we employ 30,000 people and serve over 350,000 customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as environmental and industrial process control settings. Serving customers through two premier brands, Thermo Scientific and Fisher Scientific, we help solve analytical challenges from routine testing to complex research and discovery. Thermo Scientific offers customers a complete range of high-end analytical instruments as well as laboratory equipment, software, services, consumables and reagents to enable integrated laboratory workflow solutions. Fisher Scientific provides a complete portfolio of laboratory equipment, chemicals, supplies and services used in healthcare, scientific research, safety and education. Together, we offer the most convenient purchasing options to customers and continuously advance our technologies to accelerate the pace of scientific discovery, enhance value for customers and fuel growth for shareholders and employees alike. Visit www.thermofisher.com.
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